Например:
import numpy as np
import cv2
...
color_image = cv2.imread(FILE)
gray_image = cv2.cvtColor(color_image, cv2.COLOR_BGR2GRAY)
n_image = np.around(np.divide(gray_image, 255.0), decimals=1)
[[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0.2 0.7 0.9 1. 0.9 0.7 0.4 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0.6 1. 1. 1. 1. 1. 1. 1. 0.8 0.1 0. 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0.5 1. 1. 1. 1. 1. 1. 1. 1. 1. 0.8 0. 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0.1 1. 1. 0.9 0.4 0.1 0. 0.1 0.4 0.9 1. 1. 0.4 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0.4 1. 1. 0.3 0. 0. 0. 0. 0. 0.3 1. 1. 0.7 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0.9 1. 0.8 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0.9 1. 0.6 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0.3 1. 1. 0.3 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0.2 0.6 1. 1. 0.6 0. 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0.5 1. 1. 1. 1. 0.5 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0.6 0.8 0.8 0.9 1. 1. 0.6 0. 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0.4 1. 1. 0.7 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0.4 1. 1. 0.4 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 1. 1. 0.7 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0.9 1. 0.8 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0.7 1. 0.9 0. 0. 0. 0. 0. 0. 0. 0.1 1. 1. 0.7 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0.5 1. 1. 0.5 0. 0. 0. 0. 0. 0. 0.7 1. 1. 0.5 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0.1 1. 1. 1. 0.6 0.2 0. 0.1 0.3 0.8 1. 1. 1. 0.1 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0.5 1. 1. 1. 1. 1. 1. 1. 1. 1. 1. 0.4 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0.5 1. 1. 1. 1. 1. 1. 1. 1. 0.4 0. 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0.2 0.6 0.9 1. 1. 0.8 0.5 0.1 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. ]
[0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. 0. ]]
Если округлять не нужно - то достаточно
np.divide(gray_image, 255.0)
З.Ы. Видео не смотрел